Protected Homocysteine Peptides as Precursors of Labelled Methionine Peptides. Application in Preparation of Methionine-Enkephalin.
نویسندگان
چکیده
منابع مشابه
The preparation of [35S]homocysteine thiolactone free of [35S]methionine.
The unambiguous study of homocysteine metabolism requires a source of [355]homocysteine completely free from contaminating [35S]methionine. Until now, such a reagent has not been readily available. Homocysteine is an important metabolite involved in methionine, cysteine and methylation metabolism. Homocysteine can be methylated to form methionine with either 5-methyltetrahydrofolate [1] or beta...
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A number of peptides containing methionine and glycine, such as Met-Met-Gly, Met-Met-Gly-Met, and Met-MetGly-Gly, were synthesized by conventional techniques. The growth response of Saccharomyces cerevisiae G1333, a methionine-requiring auxotroph, toward various di, tri, and tetrapeptides containing methionine was measured. The yeast mutant utilized and contained peptidase activity toward most ...
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Adrenal chromaffin granules contain at least 10 peptides, ranging in size from 3 to 5 kilodaltons, that yield, upon digestion with trypsin, peptides that show specific binding to opiate receptors. All are distinctly different from beta-endorphin. Two of these peptides have been purified to homogeneity and subjected to chemical analysis. One is apparently a [Met]enkephalin precursor containing t...
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1. A method is described that selectively purifies methionine peptides from enzymic digests of a protein. The peptides, after paper electrophoresis, are treated on paper with iodoacetamide at acid pH. This specifically converts methionine residues into their sulphonium salts. When the paper is submitted to electrophoresis at right angles to the original direction, the carbamoylmethylmethionine ...
متن کاملReversible chemoselective tagging and functionalization of methionine containing peptides.
Reagents were developed to allow chemoselective tagging of methionine residues in peptides and polypeptides, subsequent bioorthogonal functionalization of the tags, and cleavage of the tags when desired. This methodology can be used for triggered release of therapeutic peptides, or release of tagged protein digests from affinity columns.
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1985
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.39b-0157